WebApr 12, 2024 · From this RNA, DENV and CHIKV loads were quantified using 4× TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems, Foster City, CA, USA) in individual 10 μl reactions containing virus-specific primers and probes . RT-qPCR reactions were run in a LightCycler 480 instrument (Roche Applied Science, Switzerland). WebNote: (Optional) TaqMan® Fast Virus 1-Step Master Mix and the TaqMan® Gene Expression Assay can be combined ahead of time and stored at –30°C to –10°C for short periods. The assay can be added directly to the Master Mix tubes. The volumes in shipped tubes are as precise as a pipette. The target fill volume for the 1–mL tubes is 1.05 mL.
Taqman™ fast virus 1-step master mix Sigma-Aldrich
Webmaster mix vs. a 4X master mix. Using TaqMan Fast Virus 1-Step Master Mix at 4X concentration allows you to add almost twice as much sample to a reaction compared to a 2X master mix. This helps to enhance sensitivity for samples containing low concentrations of viral nucleic acids. 30 µL with 2X mix 30 µL with 4X mix Sample 11.25 µL 19.5 µL WebRev. 1 Page 1 of 2 CERTIFICATE OF ANALYSIS 4444436 TaqMan™ Fast Virus 1-Step Master Mix Packaging Lot: 00865145 Manufacturing Date: 15.01.2024 (DD.MM.YYYY) Expiration Date: 31.07.2024 (DD.MM.YYYY) QUALITY CONTROL TEST RESULT ANALYTICAL TEST TaqManTM Fast Virus 1-Step Master Mix is formulated and filled … hershey park hotel with indoor water park
TaqMan™ Fast Virus 1-Step Master Mix - Thermo Fisher …
WebTaqMan Fast Virus 1-Step Master Mix is designed for fast, highly sensitive real-time RT-PCR even in the presence of challenging PCR inhibitors. The 4X formulation provides enhanced detection of both RNA and DNA viruses and is ideal for multiplex gene expression studies, even with low target input. WebRev. 1 Page 1 of 2 CERTIFICATE OF ANALYSIS 4444434 TaqMan™ Fast Virus 1-Step Master Mix Packaging Lot: 00973647 Manufacturing Date: 07.09.2024 (DD.MM.YYYY) Expiration Date: 31.03.2024 (DD.MM.YYYY) QUALITY CONTROL TEST RESULT ANALYTICAL TEST TaqManTM Fast Virus 1-Step Master Mix is formulated and filled … WebTaqMan Fast Virus 1-Step Master Mix is designed for fast, highly sensitive real-time RT-PCR even in the presence of challenging PCR inhibitors. The 4X formulation provides … hershey park hotels package deals