Iptg x-gal spectinomycin table
WebChromoMax IPTG/X-Gal Catalog # 13988100 13988101 13988102 BP42001 BP420010 BP420050 SDS ChromoMax™ IPTG/X-Gal Catalog # 10133443 10225163 10658593 ACRBP4200 BP4200 BP42001 BP420010 BP420050 SDS 5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-GAL), Ultrapure, Thermo Scientific Catalog # ALFAAJ10077 SDS … WebStudent Guide Table 3 lists the four experimental samples (A, B, C, and Dy that you will be working with as well the conditions under which they will be grown. During the activity, …
Iptg x-gal spectinomycin table
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WebOur X-Gal and IPTG products are intended to be used together for blue-white colony screening. X-Gal (5-Bromo-4-Chloro-3-Indolyl-beta-D-Galactoside) is a chromogenic … WebPrepare 100mM IPTG solution in dH 2 O (or dilute from 1M IPTG Stock Solution). Agar Media (Recommended) 1. Cool autoclaved growth media agar to 50°C. 2. Add 10 µl X-Gal Solution** (20 mg/ml) per 1 mL of Media (or 2 µl X-Gal Solution (100 mg/ml) per 1 mL of Media). 3. Add 10 µl IPTG (100mM) per 1 mL of Media for a final concentration of 1mM. 4.
WebApr 4, 2024 · (D) The E. coli K-12 BW25113 WT and ∆ lon strains harboring the plasmid pCA24N-NHis-yefM were induced with 0.5 mM IPTG for 30 min, and 1% spectinomycin (100 µg/mL) was added to the strains to activate a stress response. We collected equivalent quantities of cells at 0 min, 30 min, 60 min and 120 min and then ran Tricine-SDS-PAGE … WebMay 18, 2024 · spectinomycin until the agar has cooled. enough to handle. However, the agar will ... colonies from the IPTG/X-gal (IX) plate. ... table below. 6. Replace the lid on …
WebLeading Life Science Research & Clinical Diagnostics — Bio-Rad WebStudent Guide Table 3 lists the four experimental samples (A, B, C, and D) that you will be working with as well the conditions under which they will be grown. During the activity, …
WebJun 4, 2015 · When expressed, the β-galactosidase enzyme can break down a dye-linked substrate called x-gal (5-bromo-4-chloro-3-indolyl-β-D-galacto-pyranoside) into galactose and an insoluble blue pigment (4-chloro-3 …
WebIPTG is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon, and it is therefore used to induce protein expression where the gene is … iras number clinical trialsWebMar 16, 2010 · Pspac = IPTG inducible promoter, PdivIVA = constitutive B. subtilis divIVA promoter used to drive expression of lacZ, AmpR = ampicillin resistance gene, SpcR = spectinomycin resistance gene. Plasmid pLK has been made available through the Bacillus Genetic Stock Centre, with accession number ECE219. iras offenburgWebTransform the reaction product to XL1-Blue and streak the transformant on Ampicillin/X-gal/IPTG LB plate. Culture O/N at 37°C. Screen for correctly assembled clones by colony PCR. You can use TALE-F/TALE-R primers both for ptCMV-153/47-VR and ptCMV-136/63-VR vectors. The detailed reaction condition is described in Appendix. order a phone from q linkWebJun 6, 2024 · The growth temperature was 37°C. The antibiotic concentrations used are as follows: 75 µg/mL ampicillin, 50 µg/mL kanamycin, 25 µg/mL chloramphenicol, 50 µg/mL spectinomycin, and 15 µg/mL tetracycline. X-gal and isopropyl β-D-thiogalactopyranoside (IPTG) were used at 25 µg/mL and 0.5 mM, respectively. order a phone numberWebSep 13, 2024 · The present invention provides a method for manufacturing an artificial polypeptide fiber, the method comprising: a step for bundling a plurality of raw material fibers containing an artificial polypeptide; and a step for bringing an obtained bundle of raw material fibers into contact with a composition containing a binder and an enzyme. iras notice of assessment 2020WebWhen spreading directly onto agar plates, we recommend 40–50 µl of 40 mg/mL X-gal (2% stock) in dimethylformamide and 30–40 µl of 100 mM IPTG on top of the agar. Let the X … order a phone lineWebchloramphenicol and spectinomycin were added at concentrations of 100 mg/ml, 10 mg/ml, 30 ... respectively. IPTG and X-gal for blue/white selection were added at concentrations of 0.1 mM and 40 µg/ml, respectively. 2.2 Constructing gRNA plasmid and donor DNA To construct gRNA plasmid, a set of primers was used to PCR amplify the pGRB backbone ... iras object to annual value