Cytiva heparin
WebAfter diluting the [NaCl] < 40 mM, prothrombin was loaded onto a 1 ml Q-sepharose column (Cytiva) attached to the bottom of a 1 ml Heparin column (Cytiva) equilibrated with 20 mM Tris, pH 7.5, 40 mM NaCl, and 10 mM EDTA. The heparin column was detached and prothrombin was eluted from the Q-sepharose column using 0.04–1 M NaCl gradient. WebCytiva's heparin resin allows for fast and reliable separations of biomolecules with an affinity for heparin, including antithrombin III antibodies, coagulation factors, and other plasma proteins. DNA-binding proteins, lipoproteins, protein synthesis factors, enzymes that act on nucleic acids, and steroid receptors can also be purified. ...
Cytiva heparin
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WebHiTrap ® Heparin HP is delivered in 0.2 M NaCl with 20% ethanol as bacteriostatic agent. Store HiTrap ® Heparin HP columns equilibrated with 25 mL of 20% ethanol or a buffer containing a bacteriostatic agent. … WebPrepacked with Heparin Sepharose High Performance, HiTrap Heparin HP columns offer fast and simple affinity purifications of proteins such as growth factors, DNA …
WebHeparin also functions as a high-capacity cation exchanger, due to its anionic sulfate groups. Gradient elution with salt is most commonly used in both cases. Heparin is coupled to Sepharose ® 6 Fast FLow with a chemically optimized linkage. Heparin is a mucopolysaccharide consisting of a repeating dimer of α-L-dipyranuronic acid 2-sulfate ... Webnative proteoglycan of porcine intestinal mucosa. Heparin consists alternating units of uronic acid and D-glucosamine, most of which are substituted with one or two …
WebImmobilized heparin is used for purification of antithrombin III, coagulation factors, and other plasma proteins. Simple operation with a syringe, a pump or a chromatography system such as AKTA design. … Webchemical stability of Capto Heparin is limited by the heparin ligand itself. Coupling is oriented to the reducing end of the heparin ligand. The ligand amount is optimized to give high selectivity for Factor IX. Fig 1. Capto Heparin, which purifies proteins with an affinity for heparin, is available in laboratory and process-scale quantities ...
WebHeparin Sepharose ® 6 Fast Flow is a well established BioProcess affinity medium designed for capture or intermediate purification steps. This medium is extensively used for industrial purification of several marketed plasma proteins. Heparin Sepharose ® 6 Fast Flow is a member of the Cytiva range of affinity chromatography media for capture and …
WebApr 6, 2024 · The T34Q-X sample (15 nmol) was desalted using a NAP-10 column (Cytiva), and the eluate was dried on a SpeedVac concentrator. The residue was dissolved in 10 mM HEPES–NaOH (pH 7.5, 100 μl), and after heating at 95°C for 10 min, the solution was loaded onto a NAP-5 column (Cytiva) equilibrated with water. inclustryWebAll. 5 × 1 mL. Price. 90100193. HiTrap Heparin Sepharose 6 FF. 5 × 1 mL. 617.44 USD. Add to cart. Add to quote list. incluvision gmbhWeb阿里巴巴Cytiva 17040701 HiTrap Heparin HP 1 x 5 ml,现货,生化试剂,这里云集了众多的供应商,采购商,制造商。这是Cytiva 17040701 HiTrap Heparin HP 1 x 5 ml,现货的详细页面。产地:Cytiva,是否进口:否,分类:诊断试剂,货号:17040701,产品规格:1 x 5 ml,品牌:Cytiva,用途范围:用于 DNA 结合蛋白、凝血因子和其他 ... incluvationsWebProduct Name. 17099801. Heparin Sepharose 6 Fast Flow. 17099803. Heparin Sepharose 6 Fast Flow. 17099825. Heparin Sepharose 6 Fast Flow. 17099804. Heparin Sepharose 6 Fast Flow. inclusões bacterianasWebCytiva's heparin resin allows for fast and reliable separations of biomolecules with an affinity for heparin, including antithrombin III antibodies, coagulation factors, and … inclut ilWebCytiva Life Sciences™ HiTrap™ Heparin HP Columns are prepacked with Heparin Sepharose™ High Performance for high-resolution purification. $280.50 - $1257.00 Specifications. Content And Storage: 4° to 30°C: Particle Size: 34 μm: For Use With (Equipment) Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ … inclutecWebGroup-specific affinity resins for purification of glycoproteins, viruses, polysaccharides, recombinant proteins, and enzymes incluyais rae